Abstract :
Background: Haemoglobin is one of the commonest tests required in pathology labs. There are many methods of haemoglobin estimation. Common methods used are sahli’s acid hematin method, photometric cyanmethemoglobin method with hemoglobin estimation by colorimeter, automated method by cell counter.
Objective: To evaluate accuracy of hemoglobin estimation by manual photometric method versus automated method by hematology cell counters using the same sample at the same time.
Materials and Methods: Blood samples of 460 adult patients and 72 children (
Results: Results by photometric method showed higher mean value compared to automated method by 2.52%. Commercial control results showed 2.2% coefficient of variation by Mindray cell counter and 2.9% by photometer.
Conclusion: Both methods are accurate with 2.52% more mean value in photometric method. When Haemoglobin is the only test required, photometric method is cost effective and feasible. If sample size is large as in tertiary care hospitals and multiple parameters like complete blood count (CBC) are needed, automated method is time effective and feasible.
Keyword :
Hemoglobin, Manual, Automated, Photometer, Cell counter.