Abstract :
Background: Cymbopogon is a member of the family Poaceae and has been explored for its phytochemicals and bioactivities. Although the antimicrobial activities of Cymbopogon flexuous oil have been extensively studied, comprehensive analyses are required to identify promising compounds for the treatment of antimicrobial resistance. Therefore, this study investigated the antioxidant and antimicrobial properties of Cymbopogon flexuous.Aim: To explore the phytochemical constituents of the oil extract of Cymbopogon flexuous.Materials and Methods: In vitro analyses were used to evaluate the antioxidant and antimicrobial properties of the Cymbopogon flexuous. In addition, bioactivity was measured using cytotoxicity assays. Antioxidant assays were performed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid (ABTS) Finally, the antimicrobial activity of these extracts was evaluated against Candida albicans, Bacillus subtilis, Staphylococcus aureus, and Escherichia coli using a well diffusion assay.Results: GC–MS analysis revealed 53 metabolites. Of these, 2,5-bis(1,1-dimethylethyl)- phenol (27.87%), alpha-cadinol (26.76%), and 1,2-dimethoxy-4-(1-propenyl)-benzene (20.56%) were the predominant compounds. C. winterianus and C. nardus exhibited the highest antioxidant activity against DPPH and ABTS, respectively. Oil extract exhibited the highest antimicrobial activity against E. coli and S. aureus, whereas Cymbopogon flexuous leaf extract showed the highest activity against microbes. Furthermore, computational pathway analysis predicted that antimicrobial activity mechanisms were related to antioxidant activity.Conclusions: These findings demonstrate that the leaves had strong antioxidant activity, whereas oil showed great antimicrobial activity.
Keyword :
Anti-oxidant assay GC.MS, DPPH, ABTS, Cymbopogon flexuous, Anti-microbial.