Abstract :
Objective: The goal of this study was to evaluated anti-glaucoma activity of ion-activated in-situ gel of Brimonidine Tartarate (BT) and
to predict the shelf of the developed formulation.
Method: sol-gel formulation was prepared by using gellan gum as an ion-activated gel-forming polymer, sterculia foetida gum and
kappa carragennan as mucoadhesive agent and hydroxy propyl methyl cellulose (HPMC E50LV) as release retardant polymer. Phenyl
ethyl alcohol as preservatives in borate buffer. Glaucoma was induced by marginal ear vein using 5% dextrose solution. Schiotz
tonometer was use to measure the induced glaucoma. Long terms and accelerated stability studies were carried out. The formulation
was characteristics for pH, clarity, sterility, in-vitro gelation studies and drug content by RP-HPLC method.
Result: The mean normotensive IOP was in the range of 17.5±0.08 to 19.1±0.40 mm Hg in both the eyes. Glaucoma was induced in the
both eye with 5% dextrose solution and the mean IOP in both eyes was in the range of 26.2±0.21 to 29.9±0.25 mm Hg. Student paired Ttest
( F2) vs. Distilled water) was analysed and it was found that there is a highly significant difference between the means. This
indicates the test drug is effective in the form of in-situ gels. The repeated measures one –way ANOVA with Tukey's multiple comparison
test indicate the data is not significant, i.e p value is greater than p
Keyword :
Sterculia Foetida gum, marginal ear vein, Schiotz tonometer, Brimonidine Tartarate.