Abstract :
A novel, simple, accurate and precise Area under curve spectroscopic method was
developed and validated for the estimation of Levofloxacin in bulk and Pharmaceutical
dosage forms. The absorption maximum at 288.6-298.6 nm in 0.1 N Sulphuric acid. The
Linearity was found to be in the concentration range of 5-30 μg/ml and the correlation
coefficient was found to be 0.999 and it has showed good linearity, reproducibility,
precision in this concentration range. The regression equation was found to be Y = 0.019
X + 0.009. The % recovery values were found to be within 99.60-101.1 % showed that
the method was accurate. The LOD and LOQ were found to be 0.2197 and 0.6591μg/ ml,
respectively. The % RSD values were less than 2. The present methods were
accomplishing the validation parameters according to ICH guidelines like accuracy,
precision, linearity, range, ruggedness, limit of detection and limit of quantitation etc. The
developed method was successfully applied for the quantitative estimation of
Levofloxacin in bulk and pharmaceutical dosage forms.
Keyword :
Levofloxacin, Area Under Curve method, 0.1N Sulphuric acid, Linearity, Precision, Ruggedness and Accuracy.