Author :
Rameshpetchi Rajendran, Devikasubramaniyan*, Ramya Sri Sura, Ch.Rakesh, D.Sangeetha, B.Shilpa, D.Nagamani
Volume :
5
Issue :
04
Abstract :
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the
validated of Dosulepin in its pure form as well as in tablet dosage form. Chromatography was carried out on Xterra
C18 (4.6×150mm, 5µ) column using a water (100% v/v) as the mobile phase at a flow rate of 1.0ml/min, the
detection was carried out at 230nm. The retention time of the Dosulepin was 2.1 ±0.02min. The method produce
linear responses in the concentration range of 5-25µg/ml of Dosulepin . The method precision for the determination
of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Dosulepin , RP-HPLC, validation.
Keyword :
Dosulepin , RP-HPLC, validation.