Abstract :
In current study the inhibitory potential of Tridax procumbens leaf extract loading chitosan nanoparticle against
calcium oxalate crystallization under in vitro condition.
Kidney stone formation was studied using three assays such as nucleation, aggregation and growth. Nucleation was
studied by adding calcium chloride and sodium oxalate solution in the presence and absence of aqueous extracts at
37ËšC. For aggregation and growth calcium oxalate monohydrate crystals were studied. The effect of extracts on the
formation and inhibition of stone forming stages were observed UV-Vis spectrophotometrically, In the present study
Tridax procumbens plant extract loaded chitosan nanoparticle were prepared via ionic gelation of tripolyphosphate
(TPP) methods synthesized nanoparticle was characterized by Scanning Electron Microscope (SEM) and Fourier
Transform infrared (FTIR).The present results showed the optimum encapsulation efficiency (77 %) was obtained
by a chitosan concentration of 0.1 mg/mL, chitosan-to-TPP mass ratio of 2 ml and Tridax procumbens plant extract
concentration of 0.8 μg/mL. Scanning Electron Microscope (SEM) imaging showed a smooth and homogenous
structure for nanoparticles. Fourier Transform infrared (FTIR) spectroscopy confirmed tripolyphosphoric groups of
TPP linked with ammonium groups of Chitosan in the Nanoparticle.The present results showed the potential of the
plant extract loaded chitosan nanoparticle in our study may provide a suitable alternative to traditional adjuvant
systems for urolithiasis.
Key words: Tridax procumbens, Chitosan, nanoparticle, loading, drug delivery system,
Keyword :
Tridax procumbens, Chitosan, nanoparticle, loading, drug delivery system,