Abstract :
Oxidative stress is considered as one of the vital factor that causes serious ailments to humans. The free radicals
speeds up the aging process and thus, the need of antioxidants gains importance as it will help to protect body from
free radicals and their threats. Due to increase in demand for antioxidant products, the present study focuses on the
formulation and evaluation of a herbal antioxidant cream from the methanol leaves extract of Polygonum minus.
Leaves was extracted by continuous hot extraction method using hexane and methanol as solvent. The extracts were
studied on its free radical scavenging activity using DPPH assay. Methanol leaves extract showed higher free
radical scavenging activity with the Inhibition Concentration range between 31.25 to 62.5 µg/ml compared to
hexane extract which is at 500 µg/ml. However, standard ascorbic acid have showed potential antioxidant activity
when compared to sample extracts. Methanol leaves extract was selected for antioxidant cream formulation due to
its phenomenal free radical scavenging activity. The invitro evaluation on the formulated cream was carried out on
several parameters such as thermal stability, pH, viscosity, type of formulation and physical appearance. Evaluation
parameters showed good results and the formulated cream was stable during the entire period of stability testing.
The formulated antioxidant cream from the methanol leaves extract of P. minus proved potential free radical
scavenging activity, which will enhance the importance of this plant to the future research community and increases
its commercial visibility in the herbal and cosmetic industry.
Keywords: Polygonum minus Huds. (P. minus), DPPH assay, IC50, Antioxidant Formulation.
Keyword :
Polygonum minus Huds. (P. minus), DPPH assay, IC50, Antioxidant Formulation.