Abstract :
Homeopathic remedies are proposed as source for traditional preventive medicines. In this context our study focuses to evaluate efficacy of homoeopathic drug with reference to GIOP in Zebrafish vertebrate model. The overall approach behind this study was to determine whether homeopathic drugs can be used along with of allopathic medicines as an alternate therapy. Methods: In the present study, an attempt is made to find out the efficacy of homoeopathic medicines- Argentum Metallicum, Calcaria Carbonica and Sepia in two dilutions 6CH and 30 CH on GIOP model of zebra fish in two dilutions. Exposure was studied at 3 different points - 4, 14 and 28 days exposure (treatment with test drugs) and staining intensities were measured using Image J software. Control groups used were untreated control larvae, GIOP model untreated post 11 dpf and GIOP model treated with Alendronate. The medium was changed every day at the same time. Staining intensities measured for Alizarin Red and Calcein dye stained images. Results: Staining intensities of Alizarin red and Calcein staining of the treated group on statistical analysis showed that the means of GIOP and other treated groups were not statistically significant at p
Keyword :
Homeopathy, Osteoporosis, Zebra fish, Prednisolone, Alizarin red, Calcein