Author :
Rameshpetchi Rajendran, Devikasubramaniyan*, Ramya Sri Sura, M. Praveen Kumar, L.Soundharya, M. Arun Kumar, M. Sumalatha
Volume :
5
Issue :
04
Abstract :
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the
validated of Entecavir, in its pure form as well as in tablet dosage form. Chromatography was carried out on a
Phenomenex Gemini C18 (4.6 x 250mm, 5µm) column using a mixture of Acetonitrile and Water (15:85% v/v) as the
mobile phase at a flow rate of 0.9ml/min, the detection was carried out at 215nm. The retention time of the
Entecavir was 3.1 ±0.02min. The method produce linear responses in the concentration range of 30-150µg/ml of
Entecavir. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the
quality control of bulk and pharmaceutical formulations.
Keywords: Entecavir, RP-HPLC, validation.
Keyword :
Entecavir, RP-HPLC, validation