Abstract :
Introduction: The effect of eugenol on white blood cells (WBCs ) and corticosteroneis known. However,
pharmacological properties and activities of eugenol such as anti-inflammatory, antioxidant, anesthetic,
muscle relaxant, improved motor coordination, and increased red blood cells were reported in stress -
induced rats.
Objective: To evaluate the effect of eugenol on WBCs in sub -acute restraint stress-induced Wistar albino
rats.
Materials and Methods: Female Wistar albino rats (n = 30) weighing (150–220 g) were divided into five
groups with six animals in each group, Group I: normal control; II : vehicle polyglycerol treated (PG); III:
eugenol treated alone - TA (150 mg/kg/i.p for 15 days); IV : stress -induced (SA-stress alone), and V: stress
followed by treated (T/S) with. Blood samples were collected at the end ofthestudyand total leucocyte count
(TLC), differentiation leucocyte count (DLC), platelet count (PC) and concentration of corticosterone were
estimated.
Results: The TA group showed significant (p
group. The platelet count was significantly less in the SA group as compared to T/ S group (p
effect of eugenol on DLC did not show a significant difference with respect to neutrophil and lymphocytes
count in T/S groups, when compared with other groups, whereas, no significant differences were observed
between basophils, monocytes, and eosinophils of T/S when compared with SA group. No significant
differences were observed in corticosterone levels in all groups.
Conclusion: Acute restraint stress induced distribution of WBC was not significantly attenuated by eugenol
and no changes were observed in corticosterone level. However, eugenol increases platelet aggregation.
Keyword :
Stress, Eugenol, Total leukocyte count, Differentiation leukocyte count, Platelets, Corticosterone.