Author :
R Venkat Reddy *, Badugu Deepika, N. Sunita, M. Jaya Shankar, SK. Nadeer, K. Ravi Kumar, Santosh Illendula, G. Koteswar Rao, K.N.V. Rao, K. Rajeswar Dutt.
Volume :
5
Issue :
04
Abstract :
A new simple, accurate, rapid, precise, reproducible and cost effective spectrophotometric method for the
quantitative estimation of Metformin in bulk and pharmaceutical dosage form. The developed visible
spectrophotometric method for the quantitative estimation of Metformin is based on measurement of absorption at
maximum wavelength 242 nm using Methanol as a solvent. The stock solution of Metformin was prepared, and
subsequent suitable dilution was prepared with methanol to obtained standard curve. The standard solution of
Metformin shows absorption maxima at 242 nm. The drug obeyed beer lambert’s law in the concentration range of
0 - 60 μg/ml with regression 0.999 at 242 nm. The overall % recovery was found to be 100.04% which reflects that
the method was free from the interference of the impurities and other excipients used in the bulk and marketed
dosage form. The low value of % RSD was indicative of accuracy and reproducibility of the method. The % RSD for
inter-day and intra-day precision was found to be 0.53 and 0.51respectively which is
Keyword :
Metformin, UV Visible Spectrophotometry, Method development, Validation, , Methanol , Accuracy, Precision.