Abstract :
Introduction: Cervical cancer is the most common cancer among females in the developing countries. HPV virus plays vital role in cervical carcinogenesis. p53 inactivation plays a key role in etiology of cervical cancer. Various screening methodologies have been developed for early detection of cervical carcinoma. Recently many biomarkers have been used for diagnostic and prognostic purpose in carcinoma cervix. p53 expression can be used as a marker for the aggressive behaviour of tumor.
Objectives: To compare the expression of p53 in inflammatory lesions of cervix and mild cervical dysplasia
Materials and Method: Patients coming to the institution who are undergoing biopsy for abnormal cytology/colposcopy during the study period of July 2015to July 2016 are included in the study. Total of 20 patients were studied. Immunohistochemistry was used to study the p53 expression in inflammatory cervicitis and mild dysplasia of cervical epithelium
Results: Out of 20 patients studied, p53 expression is found to be significant in CIN1 lesions (p value- 0.000). p53 positivity was found in CIN 1 in 38.5%(5/7) cases and negative in all cases of non- specific cervicitis
Conclusion: In the present study, p53 expression was absent in inflammatory lesions and positivity was found in CIN1 lesions. This helps in identifying the subset of dysplastic lesions which need to be followed up more vigilantly to prevent their development into cancers. This could also be used as a backup for histopathology and also in cytology when there is ambiguity in knowing whether there is dysplasia or not.
Keyword :
p53, Immunohistochemistry, Cervical intraepithelial neoplsia(CIN)