Author :
Vijayalaxmi Rajiv Shinde*, Pranav Suresh Tambe, Neha Nitin Pai, Jaspal Kaur Oberoi, Aaquil Mohamed Ashraf, Mohamed Baqir, Sathesh Challegoli
Volume :
12
Issue :
1
Abstract :
Background: Soil microorganisms play a very significant role in bioremediation process. To identify organisms which have capacity to degrade hydrocarbons in soil becomes an important approach in environmental remediation.
Aim: Screening of potential hydrocarbon degrading organisms from different soil and to evaluate its potential in environmental oil and petrol spill clean-up.
Materials and Methods: Total 4 isolates were isolated from 10 oil and petrol contaminated soil samples by using minimal media and petrol as sole source of carbon. Biochemical characterization of these isolates and physiochemical properties of soil was done. The genus of the organism was determined.
Results: Biochemical characterization of isolates was done according to key Bergey’s Manual of Systematic Bacteriology (second edition, Volume 3); the potent organism was belonging to the genus Pseudomonas. Further gene sequencing studies showed that the isolate was belonging to Pseudomonas montelii.
Conclusion: The isolated organism, identified as Pseudomonas monteilii exhibit potential oil and petrol degrading activities which make it potential candidate for environmental bioremediation.
Keyword :
Bioremediation, Pseudomonas montelli, Hydrocarbon-degrading enzymes, Bioremediation strategies, DCPIP.