Abstract :
Purpose: The purpose of this study was to determine the rate of biofilm production by S.aureus and S.epidermidis isolated from different clinical specimens.
Methods: Quantitation of biofilm formation by staphylococci was carried out using three in vitro methods namely tissue culture plate (TCP), tube method (TM), and congo red agar (CRA) method.
Results: Out of 237 (94.8%) S.aureus isolates tested, 25 strains were found to form biofilm, out of these one (0.42%) was strong biofilm producer and 24 (10.12%) were moderate biofilm producers. In TM and CRA method 20 (8.43%) and 10 (4.2%) strains were moderate biofilm producers, rest were considered as weak or non-biofilm producers. All the 25 biofilm positive strains were S.aureus.
Conclusion: The TCP method is more sensitive to detect biofilm formation by S.aureus when compare to tube method (TM), and congo red agar (CRA) method.
Keyword :
Biofilm, CRA, S.aureus, S.epidermidis, TCP, TM