Abstract :
A novel stability-indicating, RP-HPLC method was developed for the simultaneous determination of the Aspirin,
Atorvastatin and Clopidogrel in capsules. Chromatogram was run through BDS (150 mm X 4.6mm, 5μ). Mobile
phase containing phosphate buffer pH 4.5 and acetonitrile in the ratio of (50:50, v/v) was pumped through
column at a flow rate of 1ml/min. Optimized wavelength for Aspirin and Atorvastatin and Clopidogrel was 249
nm. Retention time of Aspirin, Atorvastatin and Clopidogrel were found to be 2.367 min, 5.463 min and 4.658
min, respectively. % RSD of the Aspirin, Atorvastatin and Clopidogrel were and found to be 1.01, 0.89 and 0.77
respectively. % recover was obtained as 99.79 %, 99.73 % and 100.57 % for Aspirin, Atorvastatin and
Clopidogril, respectively. Limit of detection and quantification values are obtained from regression equations of
Aspirin (0.8 ppm, 0.25 ppm), Atorvastatin (0.14 ppm, 0.41 ppm) and Clopidogrel (0.25 ppm, 0.76 ppm).
Regression equation of Aspirin is y = 2433.x + 706.4, and of Atorvastatin is y = 17432x + 872.7and
Clopidogrel is y = 2403.x + 412.3. The present method was performed the degradation studies like acid, base,
oxidation, UV, thermal and neutral, results founds to be negligible.
Key words: Aspirin, Atorvastatin, Clopidogrel, RP-HPLC and Estimation
Keyword :
Aspirin, Atorvastatin, Clopidogrel, RP-HPLC and Estimation