Abstract :
In this work we comprehensively compared the transcriptome profiles of ripe fruits (RFs) and unripe fruits
(UFs) and identified the genes related to flavonoid biosynthesis in Solanum nigrum Linn. (S. nigrum). Firstly, 118198 unigenes with a N50 length of 1339 bp were de novo assembled. In addition, 527 genes that were differential expression in RFs and UFs have been identified, including 329 down-regulated genes and 198 up-regulated genes. Especially, 8 differential expression genes (DEGs) were identified to be involved in flavonoid biosynthesis. Coincidentally, most of the 8 flavonoid-related genes were highly expressed in RFs. Finally, we found that the differential expression of three genes that were related to the synthesis of dihydroflavonol-4-reductase (DFR), flavonoid 3’, 5’-hydroxylase (F35H) and anthocyanin synthase (ANS) was the main reason for differences in flavonoid biosynthesis between RFs and UFs of S. nigrum. This study provides some supporting information for the flavonoid accumulation in RFs. Moreover, this study can improve our understanding of the molecular mechanisms of flavonoid biosynthesis in S. nigrum.
Keyword :
Transcristom; Solanum Nigrum; Next-generation Sequencing; Flavonoid; Differential Expression Genes