Abstract :
Background: Biofilm are groups of microorganism encased in a matrix of extracellular polysaccharide (slime), called polysaccharide intercellular adhesion (PIA). Bacteria commonly involved include Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The present study was performed to identify antibiotic resistance pattern & their ability to form biofilm in gram negative clinical isolates.
Material and Methods: All clinical samples received in laboratory for microbial culture during study period of 12 month (2017 to 2018) were included in this study Antibiotic susceptibility testing, ESBL & MBL detection was done for clinical isolates. Biofilm productions were determined by Congo red agar method, Christenson’s Test Tube method and Tissue culture plate method.
Result: 327 gram negative isolates were detected. Maximum were Klebsiella pneumoniae (32.72%) followed by Escherichia coli (28.44%), Acinetobacter baumanii (16.51%), Pseudomonas aeruginosa (16.51%), Citrobacter species (3.97%). Maximum isolates showed resistance to ampicillin (93.27%) followed by amoxiclave (87.46%), ceftazidime (74%). Out of 327 GNB isolates, biofilm produced by 64 (19.57%) isolates by Tissue culture plate (TCP) method, 38(11.62%) by Congo red agar (CRA) methods and 23 (7.03%) by Tube methods. Maximum biofilm were detected in Klebsiella pneumonaie (24.29%).
Conclusion: There is increase prevalence of multidrug resistant& biofilm forming bacteria. The routine monitoring of multidrug resistance pattern & biofilm detection can be recommended in clinical laboratories to guide proper antibiotic treatment.
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Keyword :
Biofilm, CLSI, Congo red agar, Test tube method, Tissue culture plate (TCP).