Abstract :
Aims: To detect the Virulence factors of extraintestinal pathogenic Escherichia coli (ExPEC). To study the antimicrobial susceptibility pattern and Extended spectrum Beta lactamase (ESBL) production by ExPEC.
Settings and Design: This is a prospective study conducted on Escherichia coli (E coli) isolated from extraintestinal sites.
Materials and Methods: A total of 150 ExPEC isolates were obtained from various clinical samples.
These isolates were tested for production of Hemolysin, Hemagglutination, Serum resistance and Gelatinase. Isolates were also tested for susceptibility to a set of antimicrobials and for production of an enzyme Extended spectrum Beta lactamase (ESBL).
Statistical Analysis: Statistical analyses were done with IBM SPSS Version 22. A “P” Value of
Results: Among 150 isolates of E coli, 53 (35.3%) were haemolytic, 66 (44%) were positive for serum resistance, 77 (51.3%) were positive for gelatinase and 78 (52%) were positive for Hemagglutination.
Antibiotic susceptibility pattern showed that maximum isolates (?90%) were resistant to Cephalosporins and Ampicillin. Least resistance (13.1%) was shown to Nitrofurantoin in cases of isolates from urine sample. Testing for ESBL production showed that majority (60.7%) of the isolates were confirmed to be ESBL producers.
Conclusion: E coli cause various extraintestinal infections because of production of virulence factors & developing drug resistance. Judicious use of antibiotics can prevent drug resistance.
Keyword :
Antibiotic susceptibility, Extended spectrum beta lactamase, Extraintestinal infections, Extraintestinal pathogenic Escherichia coli, Virulence factors.