Volume :
1
Issue :
1
Abstract :
The traditional medicinal plant ipomea pes- caprae belongs to convolvuceae family. The present study has been undertaken to find out the antioxidant activity of the whole plant extract of Ipomea pes-caprae. Plant was subjected to extraction by cold maceration by using ethanol as a solvent. Antioxidant activity such as 1,1-Diphenyl,2-Picryl,Hydrazyl (DPPH) Radical Scavenging Activity, Hydroxyl Radical Scavenging Activity, Reducing Power, Metal chelating activity were determined. Physicochemical analysis was carried out to identify the chemical constituent of the plant and showed the presence of alkaloid, sugar, steroid, glycoside, saponins, Terpenoids and phenol compounds. The result of free radical scavenging activity of EEIP by DPPH reduction revealed that the test compound is electron donor and could react with free radicals to convert them to more stable product and terminate radical chain reaction. For the measurement of reducing ability we investigated the Fe3+ to Fe2+ transformation. The metal chelating capacity of the EEIP and standard anti oxidants are determined by accessing the ability to complete with bipyridil and thiocyanate for Fe3+ and Fe2+ respectively. The formation of ferrous bipyridil, ferric – thiocyanate is not complete in the presence of EEIP. The ability of chelating is increased with increased concentration. So, it can be assumed that the plant extract chelate the iron. The experiment demonstrates that action of plant extract as per oxidation protector may be related to its iron binding ability.