Abstract :
Garcinia gummi-gutta, a tropical plant whose fruit rind is used as an herbal medicine for certain illness. However, reports on its genotoxicity and geno-protective effects are not available. Hydromethanolic rind extract of G. gummi-gutta fruit was prepared by maceration method and tested against mitomycin C, an anticancer drug. Experiments were conducted on Swiss albino mice to assess the possible dose-dependent effect of the extract (100, 200 and 300 mg/kg BW i.p). The selected doses of the extract were orally administered, and bone marrow micronucleus assay was performed. For the protective effect, the extract was orally gavaged 1 hr prior to mitomycin C (i.p. treatment -5mg/kg). Assay was performed for total antioxidant activity of the extract. Ascorbic acid was taken as the reference standard for both the assays. The extract did not induce the formation of micronucleated cells. However, the higher dose induced a significant decrease in the P/N ratio (p
Keyword :
Mitomycin C; Garcinia gummi-gutta; genotoxicity; cytotoxicity; antioxidant; genoprotection; cytoprotection