Abstract :
Introduction: Streptomycin is used in many conditions like tuberculosis, plague, tularemia and peritonitis. Kidney damage is one of the feared complications of streptomycin. Free radicals are implicated in many pathology including renal diseases. In vitro studies of aminoglycosides have demonstrated potential to generate free radicals.
Aims: The study was aimed at exploring oxidative stress induced during kidney damage by streptomycin and benefit of antioxidant vitamin C.
Materials and Methods: Total 28 Wistar male albino rats were used in the study were divided into 4 groups. In two groups saline i.m. 0.3 ml/day or vitamin C i.p.200 mcg/kg/day were given. In another two group Streptomycin 100 mg/kg/day i.m. was given either alone or along with vitamin C. Oxidative stress and generation of free radicals were measured by Serum total antioxidant status (TAS), glutathione peroxidase (GPx), glutathione reductase (GR), while blood urea and serum creatinine were measured for estimation of renal damage. Rat kidneys were examined for hisptopathology.
Statistical analysis: The data was subjected to one way ANOVA with post hoc test of significance and the values of P < 0>
Results: Administration of streptomycin led to significant fall in the values of TAS, GPx and GR while there was significant rise in the value of blood urea and s. creatinine. TAS level was elevated significantly in vitamin C treated group. When vitamin C was given along with streptomycin there was significant prevention of fall in GPx, GR and TAS while rise in blood urea and s. creatinine was prevented.
Conclusions: Free radicals are generated during treatment with streptomycin leading to nephrotoxicity which was significantly prevented by coadministration of vitamin C.
Keyword :
Streptomycin, free radicals, Glutathione peroxidase, Glutathione reductase, Total antioxidant status, Nephrotoxicity.