Volume :
2
Issue :
4
Abstract :
Propionibacterium acne bacteria live deep within follicles and pores, away from the surface of the skin. In these follicles, P. acnes bacteria use sebum, cellular debris and metabolic byproducts from the surrounding skin tissue as their primary sources of energy and nutrients. Elevated production of sebum by hyperactive sebaceous glands (sebaceous hyperplasia) or blockage of the follicle can cause P. acnes bacteria to grow and multiply. The Medicinal herb of Ocimum basilicum (Lamiaceae) leaf, seed and stem was screened for its Macroscopical, Physiochemical parameters, Florescence analysis (Day light, and long UV), The dried leaf powder (15gms) was extracted by Cold maceration with 70% alcohol. The percentage value of extracts is 7.38. The dried seed and stem powder material 25gms was subjected to soxhlet extraction with 99% ethanol for continuous hot extraction for 4 hours separately. The extracts were concentrated under reduced pressure to obtain the extract solid restudies. The percentage value of the extract was 19.75% and 8.375. These extracts were qualitatively screened. The qualitative chemical analysis is made by based upon the color reaction; Alkaloids, Amino acids, flavonoids, Phenolic groups, saponins, and Tannins were commonly present in each extract of Ocimum basilicum. Further confirmed by thin layer chromatography also performed depending upon the mobile phase and detecting agents were used. The plant of Ocimum basilicum 70% ethanolic leaf extract, 99% ethanolic seed and stem extracts effective against the Micro organisms of propionibacterium acne by disc diffusion method. The extract of leaf, seed and stem of Ocimum basilicum showed highest activity at minimum concentration. The Plant extracts having marked activity against the microorganism tested in dose dependent manner.