Volume :

3

Issue :

4

Abstract :

A simple, accurate, rapid, precise and novel Reverse phase High Pressure liquid chromatographic method (RP-HPLC) has been developed and validated for simultaneous determination of Meclizine & Nicotinic acid in pharmaceutical dosage form.  max of Meclizine was 220 nm and Nicotinic acid was 273 nm. The selected and optimized mobile phase was acetonitrile: potassium dihydrogen phosphate buffer (0.02M, pH 3.0) (55:45v/v) and conditions were flow rate (1.0 ml/minute), wavelength (234 nm), Run time was 20 min. The retention time were found to be 3.01 min and 6.07 min for Meclizine & nicotinic acid respectively. Linearity and range was found to be 0-140 µg/ml for Nicotinic acid and 0-150 µg/ml for Meclizine. The proposed chromatographic conditions were found appropriate for the quantitative determination of the drugs. The method was validated for accuracy, precision, specificity, linearity, robustness, sensitivity, LOD and LOQ. The proposed method was successfully used for quantitative analysis of tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that method is specific, rapid, reliable, and reproducible.