Abstract :
Introduction: An improved highly sensitive and robust reversed-phase high-performance liquid chromatography (HPLC) method for simultaneous determination of atazanavir (ATV), efavirenz (EFV), lopinavir (LPV), and ritonavir (RTV) along with carvedilol (IS) as an internal standard. Materials and Methods: Shimadzu HPLC system (Tokyo, Japan) containing a LC10AD and LC10 ADvp with UV detector (SPD-10A) and Thermo Hypersil C18 column was used in chromatography. The mobile phase comprising acetonitrile (ACN): 10 mM potassium di-hydrogen orthophosphate (55:45) delivered at a flow rate of 1 mL/min and monitored at a wavelength of 210 nm. The method developed was optimized using central composite design, a chemometric tool to optimize the factors (ACN, buffer concentration, and flow rate) effecting and interacting with the responses (k1, Rs2,1, Rs3,2, and tR5). Results and Discussion: The analysis time is 5.8 (
Keyword :
Atazanavir, carvedilol, central composite design, efavirenz, high-performance liquid chromatography, lopinavir, ritonavir