Abstract :
Background and Objective: Osteosarcoma is an unusual bone cancer with high-grade malignancy which
generally affects adolescents and young adults. Osteosarcoma is usually treated by preoperative and
postoperative chemotherapy and surgery. Several risk factors may involve in development of osteosarcoma
cancer, among them the association of BK virus with osteosarcoma have been already investigated. Thus we
aimed to determine the association of BK and HBV virus among the patients with osteosarcoma.
Methods: Fifteen formalin-fixed paraffin-embedded tissues samples of patients with osteosarcoma including 10
male and 5 female were collected. After DNA extraction from each sample, Polymerase Chain Reaction (PCR)
was performed for detection of BK DNA virus and Hepatitis B DNA Virus (HBV).
Results: Three out of fifteen (20%) participants consisting of 2(13.3%) male and (6.66%) female showed
positive for BK virus by PCR. All the 3 patients were high grade primary osteosarcoma with mean age of 13 to
19 year old. NO HBVDNA was detected in the patients with osteosarcoma.
Conclusion: Although high prevalence of BK, about 20%, was observed among the patients with osteosarcoma,
more comprehensive studies are needed. To manage and improve treatment the screening of BK virus DNA
should be implemented for all the osteosarcoma patients.
Keyword :
Osteosarcoma, BK virus, Hepatitis B Virus, Polymerase Chain Reaction