Abstract :
The study was undertaken to evaluate the relative efficiency of doubled haploid production methods following the chromosome elimination approach utilizing maize- and Imperata cylindrica- mediated systems. For doubled haploid production, triticale x wheat derived recombinants in different generations comprised of F1, F2, F3, F4, BC1F1, BC1F2, BC1F3, BC1F4 and BC2F3 were used. The haploid embryos were cultured in the MS medium with different mineral constituents and hormones. All the generations responded for different haploid induction parameters following maize- and Imperata cylindrica- mediated systems. Advanced generations responded better than early generations for haploid induction through both systems. All the haploid induction parameters, Imperata cylindrical- were performed significantly better than maize.
Keyword :
Double haploid, Zea mays, Imperata cylindrica, Triticale x wheat derived recombinants