Volume :

3

Issue :

4

Abstract :

A Simple, accurate and rapid RP-HPLC method has been developed for the estimation of doxycycline (DOXY) in bulk and pharmaceutical dosage forms using an Altima C 18, 150 x 4.6 mm i.d, 5 µm particle size in isocratic mode; with mobile phase comprising of buffer (0.1% of potassium dihydrogen phosphate) and acetonitrile in the ratio 60:40 (v/v). The flow rate was 1 ml/min and detection was carried out by photodiode array detector at 268 nm. The retention time for DOXY was found to be 2-897 min. The proposed method has permitted the quantification of DOXY over linearity in the range of 25 – 150 µg/ml and its percentage recovery was found to be 99.134 – 101.997 %. The % RSD of intraday and inter day precision were found 0.7999% and 1.3%.