Volume :

3

Issue :

1

Abstract :

A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form. Chromatographic analysis was performed on a Symmetry X-terra C8 (4.6mm x 150mm,5m) column at ambient temperature with a mixture of mixed ortho phosphoric acid buffer and Acetonitrile in the ratio 50:50 v/v OPA buffer ; as mobile phase, at a flow rate of 0.9 mL min-1. UV detection was performed at 260 nm. The method was validated for accuracy, precision, linearity and sensitivity. The retention times of Etodolac and Paracetamol were 2.190 and 3.373 min, respectively. Calibration plots were linear over the concentration ranges 4–20 μg mL-1 and 5-25μg mL-1 for Etodolac and Paracetamol, respectively. The Limit of detection was 0.361 and 0.559µg mL-1 and the quantification limit were 1.203 µg mL-1 and 1.864µg mL-1 for Etodolac and Paracetamol, respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.23% to 99.93%. Commercial tablet formulation was successfully analyzed using the developed method and the proposed method is applicable to routine analysis of determination of Etodolac and Paracetamol in pharmaceutical tablet dosage form.