Abstract :
OBJECTIVE: Research objective was the determination of the Zinc Sulphate role as an agent of hepatoprotective in the
acetaminophen-induced changes of histopathological nature in the model of animals.
DESIGN: Research was Observational Experimental in nature.
SETTING: Research was carried out in the Pathology and Pharmacology Department Mayo Hospital Lahore from Dec, 2016 to
Mar, 2017.
METHODOLOGY: Our research sample was 90 albino rats with the range of weight from 18 – 32 grams and we made three
groups each group consisting of thirty albino rats. Control group was A group and normal saline level was maintained in this
group as 0.9%; acetaminophen was given (250 mg / kg) in B group through a single dose; Zinc Sulphate (1 – 5 mg / kg) was
given to C group for a duration of 1 – 7 days before the acetaminophen (250 mg / kg) Sigle dose. After the six hours’ duration
was conducted biochemical studies after giving acetaminophen to albino rats. At last step of the treatment weight of all animals
was checked at than sacrificed. Histopathological and gross examination was carried out for liver and statistical evaluation of
the data was carried out through Chi-Square test.
RESULTS: Demonstration of the zinc’s protective effect was made through serum concentration reduction level of the enzymes
of liver such as aspartate aminotransferase, lactate dehydrogenase, alanine aminotransferase and serum sorbitol dehydrogenase,
including the histopathological centrilobular congestion changes, necrosis and hepatocellular degeneration. We observed
through the histopathological evaluation that typical changes in pathological environment of centri-zonal necrosis, leukocyte
infiltration, steatosis, edema in animals and portal tiraditos those were treated only with acetaminophen. Animal pretreatment
through zinc sulphate lead the whole procedure to dependent on the dose for the avoidance of various changes.
CONCLUSION: It is concluded that Zinc is responsible for the production of hepato-protective effect with the prevention of
ultrastructural hepatic tissue injuries and also causes free amino acid metabolism disturbance which is a result of the
acetaminophen toxic dose.
Keywords: Acetaminophen, zinc, liver toxicity and hepato-protective effect.
Keyword :
Acetaminophen, zinc, liver toxicity and hepato-protective effect.