Volume :

2

Issue :

4

Abstract :

Background and Objectives: Aerobic, nonfermenting gram-negative bacilli (non-fermenters) are now emerging as important uropathogens. This study was done to know the prevalence of non-fermenters in Urinary Tract Infections (UTI) along with their antibiotic sensitivity. Method: 1361 mid stream urine specimens received in 18 months period from December 2012 to July 2014 were subjected to Gram, staining, semi-quantitative culture, biochemical tests and antibiotic sensitivity testing as per the standard procedures in the routine microbiology laboratory and the results were noted. Results: Out of the 317 culture positive cases with significant bactereuria, 131 (9.7%) were caused by non-fermenters. Among the non-fermenters, Pseudomonas spp. was the most common (80.6%) followed by Acinetobacter spp. (19.3%). Majority of patients belonged to the age group of 15 to 50 years and most of them were females. Antibiotic sensitivity testing revealed that, among Pseudomonas spp., 77.4% isolates were sensitive to Piperacillin, 71.13% sensitive to Amikacin, 41.9% sensitive to Ciprofloxacin, 35.4% sensitive to Ceftazidime and 90% sensitive to Imipenem. In the case of Acinetobacter spp., 68% isolates were sensitive to Amikacin, 33.3% sensitive to Norfloxacin and all were sensitive to imipenem. Out of the 6 non-fermenter isolates which were resistant to commonly used antibiotics, 4 were from inpatients indicating the possibility of nosocomial infection. Conclusion: The non-fermenters are emerging as important cause of Urinary Tract Infections. In this study, Pseudomonas spp. was the most common non-fermenter isolated in significant numbers in MSU specimens. But, Acinetobacter spp. appeared to be more resistant to invitro antibiotics in general.