Abstract :
Background and Objectives: Nonfermenting gram-negative bacilli (NFGNB), are a taxonomically diverse
group of aerobic, nonsporing, bacilli that are saprophytic in nature. They have emerged as an important
healthcare-associated pathogens. They have exhibited resistance clinically to a variety of antibiotic groups.
Thus, the present study was conducted to isolate, identify and carry out in vitro susceptibility antibiogram
of NFGNB from various clinical specimens and also to highlight their clinical significance among the
in-patients admitted at Basaveshwar Teaching and General Hospital, Gulbarga, Karnataka.
Materials and Methods: 120 isolates from various age groups of both male and female patients were
included in the study. A detailed history was elicited and the clinical specimens were collected under
aseptic precautions and subjected to preliminary biochemical test and further speciation was done.
Results: NFGNB were most frequently isolated from local infections, with Pseudomonas aeruginosa,
Acinetobacter baumanii and Pseudomonas fluorescens, being the more common. These species showed
variability in their antibiotic susceptibility results, most of them exhibiting resistance to Penicillin group of
drugs and high sensitivity to Carbapenem group of drugs.
Conclusion: P.aeruginosa had shown good sensitivity to imipenem, carbenicillin and of loxacin. A.
baumannii showed good sensitivity to imipenem, amikacin and gentamicin. As, the sensitivity pattern
varies from hospital to hospital and population to population, it is essential to establish the clinical relevance
of the isolated NFGNB. This would avoid unnecessary usage of antibiotics and emergence of drug-resistant
strains.
Keyword :
Non fermenting gram negative bacilli, Antibiotic susceptibility, antibiogram, Ps. aeruginosa, Ac. baumanii, Ciprofloxacin, Ofloxacin, Imipenem.