Preparation, optimization and evaluation of liposomes encapsulating diclofenac sodium and charge inducers to enhance stability using lipid hydration method.

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Aims The aim of the study is to encapsulate, optimize and characterize the liposomal preparations of various formulations of Diclofenac sodium (DS) along with phosphatidylcholine, cholesterol, stearylamine and dicetylphosphate. Settings and design Rotary evaporator is set at a temperature of 400C with constant rotation speed. Instruments used during the study includes Whirl mixer, Hellos software, zetasizer, Ultracentrifugation, Dialysis tube and UV Spectrophotometer. Methods and Material Diclofenac Sodium, Phosphatidylcholine, Cholesterol, Dicetylphosphate, Stearylamine, Phosphate buffer and solvents. Liposomes were prepared by Lipid-Hydration technique using rotary evaporator (RE-300). The prepared liposomes were analyzed for size, zeta potential, percentage of drug encapsulated, in-vitro drug release and stability studies. Results Particle size of the drug loaded liposome was decreased when compared to that of the drug free. Encapsulation efficiency of the drug loaded liposomes with PC shows increase in the percentage of drug encapsulated to that of the lower concentrated vesicles and positive charge inducer have revealed elevated encapsulation efficiency. Liposomes composed of PC: CHOL: SA observed to be released at high rate and stability studies confirms that PC: CHOL: SA is supreme stable at varied temperatures. Conclusions Phosphatidylcholine, cholesterol and stearylamine based preparations posses the suitable % drug encapsulated and release rate. The composition PC: CHOL: SA at a concentration of 16:8:4 Âµmoles proved as a stable suspension. From the study it can be concluded that cholesterol and stearylamine based phosphatidylcholine liposomes are most suitable to encapsulate the Diclofenac sodium.