Abstract :
Naturally found plants have become the boon to the field of herbal science and medicine. For the detection of bioactive principles present in the medicinally important plants, a preliminary screening of the phytochemicals is the valuable step which may lead to drug discovery and development. In this study, chief phytoconstituents from the leaves and roots of Calotropis procera were identified and further antimicrobial activity was studied. Screening of Calotropis procera was performed for the presence of alkaloids, carbohydrates, saponins, phenols, flavonoids, tannins, anthocyanins, proteins and terpenoids using standard methods. It was found that all the three extracts showed the presence of carbohydrates and saponins. Alkaloids, proteins and tannins presence was seen in methanol and acetone extracts. Further, flavonoids presence was seen in the methanol and aqueous extracts. The aqueous, methanolic, acetone solvents extract was prepared to test antimicrobial activity taking E. coli. The prepared extracts were subjected to antimicrobial activity against E. coli using agar well diffusion method where Polymyxin-B for E. coli was taken as a control. Methanol, acetone and aqueous extract showed a zone of inhibition of 9.5mm, 4mm and 6mm respectively.
BY Sagar Bashyal | Shubham Rai | Osama Abdul Manan | Faiz Hashmi | Avijit Guha"Preliminary Phytochemical Testing and Antimicrobial activity of Calotropis procera leaves."
Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017,
URL: http://www.ijtsrd.com/papers/ijtsrd7104.pdf
Paper URL: http://www.ijtsrd.com/biological-science/biotechnology/7104/preliminary-phytochemical-testing-and-antimicrobial--activity-of-calotropis-procera-leaves/sagar-bashyal
Keyword :
Calotropis procera, Solvent extraction, phytochemical analysis, antimicrobial activity.