Abstract :
The present investigation was carried out to quantify the phytochemical constituents such as total flavonoid,
total terpenoid and anti-inflammatory activity of aqueous stem extract of Salacia oblonga. Preliminary screening
involved the qualitative methods to detect the availability of phenol, tannin, saponin, alkaloids, terpenoids, quinones,
flavonoids, coumarins, steroids, glycosides etc. Total flavonoid and total terpenoids were quantitatively estimated.
Since flavonoids and terpenoids have potential anti-inflammatory activity. Total flavonoid and total terpenoid
contents of aqueous stem extract were found to be 19.82 mg quercetin equivalents per gram and 96.2 mg per gram
respectively. In vitro anti-inflammatory activity of aqueous stem extract of S.oblonga was evaluated by albumin
denaturation membrane stabilization tests. The percentage inhibition of denaturation at a concentration of 50 mg/ml
of extract was 97.5% and the percentage inhibition of haemolysis shown 89.14%. From the result it was concluded
that the availability of secondary metabolites in the stem extract of S.oblonga may be responsible for the antiinflammatory
activity. Therefore our study supports the isolation and herbal usage of S.oblonga stem for treating
inflammation.
Keyword :
albumin denaturatio, anti-inflammatory, membrane stabilization, Salacia oblonga, terpenoids