Abstract :
Background: Dengue virus is a member of arbovirus, family flaviviridae, genus flavivirus. These are found in common tropical areas of the world or the transmitted through bite of the female mosquito Aedes aegypti and chiefly occurs during the rainy season. It is classified into three parts Dengue Fever, Dengue hemorrhagic fever, Dengue shock syndrome. The signs of dengue: fever, headache, retro orbital pain, muscles pain, joints pain or rashes are usually seen on 3-7 days. DF is also called break bone fever.
Aim & Objective : To study the occurrence of dengue infected patients. To determine the frequency of dengue patients presenting with fever of less than 5 days in NS1 Antigen ELISA. To determine the frequency of dengue infected patient presenting with fever for more than 5 days in IgM ELISA.
Materials and Methods: The study was carried out from period of 2019 to 2021. Blood samples were collected from 161 patients. Serum was separated from blood samples. NS1 Ag detection was done from ELISA method & IgM Ab was detected by using Dengue MAC Capture ELISA test.
Result : A total of the 161 blood samples were tested, 85 (53%) were positive & 76 (47%) were negative for dengue. The dengue infection was commonly found in males than females, the common age groups of patients presenting with dengue was 11-30years.
Conclusion: Our study focused on continuous monitoring and community action for individuals and dengue controls. This research will help to improve the ways of surveillance, prevention and control.
Keyword :
ELISA, DF, Dengue MAC Capture ELISA