Volume :
2
Issue :
1
Abstract :
Two simple, sensitive and economical spectrophotometric methods were developed for the determination of ampicillin trihydrate in pharmaceutical formulations. Method A is based on the formation of pink colored complex by the reaction of drug with ferric chloride and 2, 2 bipyridyl, which absorbs maximally at 500 nm. Method B is based on the formation of orange colored complex by the reaction of drug with ferric chloride and 1,10 phenanthroline, which absorbs maximally at 523nm.Beer’s law is obeyed at a concentration range of 1-10 and 1-12 mcg/ml for method A and method B. Both the methods have been successfully applied for the assay of the drug in pharmaceutical formulations.