Volume :
2
Issue :
1
Abstract :
Analytical method development and validation play important roles in the discovery, development and manufacture of Pharmaceuticals. A simple and reproducible UV- spectrophotometric method for the quantitative determination of Lamivudine in Tablet formulation was developed and validated in the present work. The parameters linearity, precision, accuracy was studied according to ICH guidelines. Lamivudine has the maximum wavelength at 275 nm. Analytical Calibration curves were linear within a concentration range from 4.8 to 7.2µg/ml. The developed method was applied directly and easily to the analysis of the pharmaceutical tablet preparations. %R.S.D was found to be 0.475 (Lamivir HBV 100 mg Tablet) respectively. The result of analysis has been validated statistically. Hence the proposed method can be used for the reliable quantification of Lamivudine in Tablet formulation.