Volume :
2
Issue :
1
Abstract :
A Simple, specific and sensitive an isocratic Estimation by RP-HPLC analytical Method were developed and validated for the quantification allopurinol and alpha lipoic acid. Quantification was achieved by by using the mobile phase (Phosphate buffer Ph3.5: Acetonitrile) in the ratio of 55:45. Inertsil ODS, C-18,250×4.6mm ID, 5µm Particle size was used as stationary phase. The flow rate was 1.2 ml/min. Measurements were made at a wavelength of 230nm. The average retention times for Allopurinol and alpha Lipoic acid was found to be 2.457 & 6.320 min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear from 60-140µg/ml & 60-140 µg/ml for Allopurinol and alpha Lipoic acid respectively. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Allopurinol and alpha Lipoic acid.