Method development and validation for estimation of rufinamide in tablet dosage forms by RP-HPLC


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Article type :

Original Article

Author :

Gandla Kumaraswamy, Repudi Lalitha, K. Vijaypraksh

Volume :

3

Issue :

2

Abstract :

A simple, precise, rapid and reproducible stability indicating RP -HPLC method was developed and validated for the determination of Rufinamide in pharmaceutical dosage forms. Chromatography was performed on a C18 (ODS) Acetonitrile: water: Triethylamine buffer pH4.6: Methanol (70:20:10 v/v) was used as mobile phase and flow rate was adjusted to 0.8 ml/ min. The detection was carried out at 292 nm using analytical Tech. UV-Visible detector SpD-10AVP. The obtained calibration curve was linear in the concentration range of 10–50ug/ ml. The limit of detection and quantification was found to be 1.056 ug/ml and 3.09 ?g/ml respectively. Key words: Rufinamide, Reverse phase -HPLC, PDA, and Tablet dosage forms