Abstract :
Three new metal complexes derived from the reaction of Cu(II), Ni(II) and Zn(II) acetates with the Schiff-base
ligand (HL) resulted from the condensation of the 1,2-Di(2-thienyl)-1,2-ethanedione (Thenil), hydrazine hydrate
and 2-Hydroxy-5-methylbenzaldehyde have been synthesized. The structure of the synthesized ligand and
complexes was characterized by, elemental analyses, Mass, IR, UV–Vis., measurements. The binding properties
of these complexes towards DNA have been investigated using electronic absorption, competitive fluorescence
titration, viscosity measurements and circular dichroic analyses. The results obtained indicated that the
complexes get bonded with DNA via an intercalation binding mode with an intrinsic binding constant, Kb 5.01 x
104 M-1
, 4.68 x 104 M-1 and 4.21 x 104 M-1 and Kapp of 5.82 x 105 M-1
, 4.97 x 105 M-1 and 4.29 x 105 M-1
Keyword :
Thenil, DNA binding, methyl salicylaldehyde, Intercalation, hydrazine hydrate.