Volume :

3

Issue :

4

Abstract :

A simple, sensitive and precise reverse phase high performance liquid chromatographic method has been developed for the estimation of Aspirin and Clopidogrel in pure sample and its pharmaceutical dosage form. The mobile phase consists of Phosphate buffer (0.02 M Potassium dihydrogen phosphates, pH-3 adjusted with ortho phosphoric acid): acetonitrile in the ratio of 65:35 v/v delivered at a flow rate of 1.0 ml / min and wavelength of detection at 229 nm. The retention times of Aspirin and Clopidogrel were 2.673 and 3.627min respectively. The developed method was validated according to ICH guidelines. The result indicates that the method was found to be simple, rapid, and accurate and can be adopted in routine analysis of Aspirin and Clopidogrel in Pure sample and its Pharmaceutical dosage forms.