Abstract :
Among the marine organisms, Actinomycetes had gained special attention due to its major role in recycling organic matter, production of wide varieties of bioactive compounds like enzymes, antibiotics etc. In the present study Actinomycetes producing L-asparaginase activity were isolated from marine sediments of Muttukadu. Nineteen isolates were obtained and among 19, three isolates viz., MK1, MK15 and MK 16 showed maximum asparaginase activity. The enzyme extracted from the isolates was partially purified by salting out with ammonium sulphate precipitation, followed by sephadexG200 filtration. The isolate MK 16 showed high specific activity of 65 IU/mg.
Keyword :
Marine ecosystem, Actinomycetes, Therapeutic enzymes