Abstract :
MRSA infections have been confined to health care centres for decades, which is recognised as one of the major pathogen in Hospital as well as community settings. MRSA infections are associated with high morbidity and mortality. However, nowadays MRSA infections are increasingly seen in young healthy individuals with no exposure to health care centres, ie;CA-MRSA infections are seen increasingly. 50% to 70% of Staphylococcus aureus isolates submitted to area labs are methicillin resistant. MRSA is a major threat to patient safety. There are antibiotics that can kill MRSA germs. MRSA are resistant to most of the beta lactam antibiotics, not only to beta lactam but also to several other agents which is used for treatment. Imipenem is the most important antibiotic of Carbapenem, it is bactericidal for most aerobic and anaerobic pathogenic bacteria except MRSA. But in several studies its action against MRSA was shown. Here in this study, invitro susceptibility testing indicated that Imipenem is active against MRSA at different conditions, ie; at a physiological pH 7.4 & pH 5.5 an abscess condition and at two temperature 370c and 300c .Since the methicillin resistance is enhanced at 300c, the MIC of Imipenem detected at two temperature. Imipenem has a low molecular weight so it has a better penetration effect, than vancomycin and Daptomycin which is used for the treatment of MRSA. In, invitro study Imipenem spread in the medium more rapidly, and kill bacteria. So in MIC testing by Epsilometri test there is wide zone will be seen.
Context: Imipenem is the most important antibiotic of Carbapenem, it is bactericidal.
Aims: To detect the in-vitro susceptibility of Imipenem by E test in different conditions of Methicillin Resistant Staphylococcus aureus.
Settings and Design: Malabar Institute of Medical Sciences, Calicut; Randomized controlled trial.
Materials and Methods: Sample collection, Confirmation of MRSA by latex agglutination and cefoxitin e-test, detection of MIC of Imipenem by E-test at different conditions of MRSA, Detection of combination effect of Imipenem and Daptomycin against MRSA by E-test.
Statistical analysis used: paired sample t test
Keyword :
MRSA, Imipenem, Different pH of 5.5&7.4, Temperature 30&370c, Daptomycin