Abstract :
Phytochemicals show increasing demand in the market with their potent antioxidant, immune stimulant,
growth promoting, anti-pathogenic, and anti-stress activity. Phytochemical constitution, antioxidant potential,
antibacterial and antifungal activity of solvent extract (Aqueous, methanol, ethanol, ethyl acetate) of Amorphophallus
paeonifolius tuber was studied. The phytochemical constituents and their composition in solvent extracts of the tuber
from Amorphophallus paeonifolius were quantitatively estimated. DPPH, free radical scavenging activity, phosphor
molybdenum assay, ABTS radical scavenging property, and ferric ion reducing antioxidant power assay were studied
to measure the antioxidant properties of extracts of the tuber. Antibacterial activity was performed for four bacterial
pathogens (both gram positive and gram negative) using DMSO as a solvent. In phytochemical estimation, phenolic
and flavonoid contents were highest in methanolic extract (2.5±0.100 mg GA/100 mg extract and 10.7±0.6 mg
rutin/100mg extract) respectively. In ethanolic extract tannin content (91.72±0.91 mg tannic acid/100mg extract). In
antioxidant activity, ethanolic extract showed maximum activity for DPPH (53.01% at 40 µg/ml), hydroxyl radical
(100%), and 56.03% of ABTS radical cation scavenging activity. Among four solvent extracts, aqueous extract
exhibit maximum antibacterial activity for Streptococcus aureus – MTCC 737 (3.25 mm zone of inhibition),
Klebsiella pneumonia - MTCC 109 (0.75 mm zone of inhibition), Pseudomonas aeruginosa - MTCC 424 (1.25 mm
zone of inhibition) and 1.5 mm of zone of inhibition for Bacillus subtilis - MTCC 121. In phytochemical screening,
presence of flavonoids, phenols, tannins, the tuber extracts may be used as anti-allergenic, anti-cancer, antipathogenic
agents and used to treat cardiovascular disease.
Keyword :
Amorphophallus paeonifolius, phytochemicals, antioxidant, DPPH, ABTS, antibacterial.