Volume :
1
Issue :
4
Abstract :
In vitro methods play important role for the preclinical studies for any activity, which makes support to the in vivo studies. Cynodon dactylon (L.) Pers (Gramineae family), possesses various medicinal property, Piper betle belongs to the family piparaceae. In the South East Asia region, Piper betle L. are among the plants that have been associated with the control of caries and periodontal diseases. The dried materials were coarsely powdered using an electric blender. Powdered materials (500g) were then packed in soxhlet apparatus and successively extracted with petroleum ether, Benzene, chloroform, ethyl acetate, methanol and water. Each time before extraction with the next solvent, the powdered materials were dried in hot air oven at below 50ºC. About 2 gms of the concentrated ethyl acetate extract was mixed with suitable quantity of silica gel (100-200 mesh). The elute was concentrated by evaporating the solvent and the residues named as CD isolate (90% of Chloroform: 10 % of Ethyl acetate) and PB isolate (50% of Toluene: 50 % of Ethyl acetate) were identified by High performance Thin Layer Chromatography. The selected isolates from Cynodon dactylon and Piper betle and the combination of both were found to have antioxidant activity in different models in the present study. The antioxidant activity of these isolates might be due to inactivation of free radical or complex formation with metal ion, or a combination of both. α-Amylase catalyses the hydrolysis of α-1,4-glucosidic linkages of starch, glycogen and various oligosaccharides and α-glucosidase further breaks down the disaccharides into simpler sugars, readily available for the intestinal absorption. The result indicate that the selected isolate posses better antioxidant and alpha amylase inhibitory activity in combined form when compare to individual form.