Abstract :
Immobilization of bile acid enzymes named, 3-alpha hydroxysteroid dehydrogenase (3-a HSD) and lipoyl
dehydrogenase (Diaphorase) enzymes was done on to activated polymeric membranes like amino-ethyl
cellulose and Polyvinyl Alcohol. Immobilization of bile acid enzymes was done onto activated aminoethyl
cellulose by covalent coupling by using NaOH and glutaraldehyde. And, Immobilization of bile acid
enzymes was done onto activated polyvinyl alcohol by using terephthaladehyde and 2-(m-aminophenyl)-
1-3-dioxolane. Percent of enzyme coupled was found 65% and 60% for enzymes coupled onto activated
amino-ethyl cellulose and activated polyvinyl alcohol respectively. Observed percent of analytical recovery
for bile acid estimation was
% of retention activities were observed for activated polymeric membranes bound bile acid enzymes in our
present method even after the regular use of more than 4 months (16 weeks) when preserved at 4 dgree C.
Hence, our present method of immobilization of bile acid enzymes onto activated polymeric membranes
were found to be more cost effective as compared to other reported enzyme based methods.
Keyword :
3-alpha hydroxysteroid dhydrogenase, Lipoyl dehydrogenase, Bile acid, Amino-ethyl cellulose, Polyvinyl Alcohol.