Abstract :
Introduction: Correct identification of Candida species is necessary for actual therapy and epidemiology study. Candida species is the most frequent emerging cause of fungal infections globally. The most common cause of Candidemia worldwide is Candida albicans, however, non-Candida albicans (NCA) species like C. tropicalis, C. glabrata, C. parapsilosis, C. krusei and C. guilleirmondii being reported Aim and Objective: To identify the different species of Candida from blood specimen of neonates by
PCR-RFLP technique.
Materials and Methods: Total 27 Candida isolates were collected from blood samples of neonates. All the clinical specimens were inoculated for the isolation of Candida species using standard mycological techniques. The isolates were cultured on Sabouraud’s Dextrose Agar (SDA) and further, they were identified by PCR and RFLP method.
Result: Molecular methods like PCR-RFLP were used in identifying six important Candida species (C. tropicalis, C. albicans, C. parapsilosis, C. glabrata, C.krusei and C.guilliermondii) from blood. By using universal primers ITS-1 and ITS-4, PCR assay successfully amplified the ITS-1 and ITS-2 region of 27 isolates and the amplicon size of approximately 510-870 bp. This method is speedy, easy, and affordable which can be used in routine laboratory diagnostics for the quick isolation of Candida species from blood samples.
Conclusion: It is helpful for clinicians to start actual therapy in Candidemia patients before antifungal sensitivity results are obtainable.
Keyword :
Neonates, Candida, Candidemia, PCR-RFLP