HPTLC finger print profile of n-hexane extract of Rauwolfia tetraphylla Linn

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Abstract :

The objective of the present study is to evaluate the phytochemical composition and HPTLC finger print profile of medicinally useful plant R. tetraphylla L. (Apocyanaceae) leaf n-hexane extract. The CAMAG HPTLC system was used for the finger print profiling using the mobile phase toluene: ethyl acetate: acetic acid (55:45:1 v/v). The profile showed that the leaf extract of R. tetraphylla exhibited several peaks with different Rf values when visualized. At 254nm and 366 nm .At 254 nm a total 14 peaks were observed and at 366nm 10 peaks were observed. The HPTLC profile of R. tetraphylla leaf n-hexane extract is used to identify the number of chemical components and their concentration; this in turn helps to identify the chemical constituents in medicinal products and also in identification of adulterants in medicinal products mainly herbal medicine.