Abstract :
In the present study, a simple, precise and accurate high performance liquid chromatography with photodiode array
detector was developed for the simultaneous estimation of ivermectin & fenbendazole in bulk and tablet dosage
forms. A Zorbax C8 column (250 cm × 4.6 mm × 5 μm) with mobile phase consisting of 0.1 M potassium dihydrogen
orthophosphate and methanol (60:40 v/v) having pH 4.5 (adjusted with orthophosphoric acid) was used. The flow
rate was 1.2 ml/min and the effluents were detected at 268 nm. The retention times of ivermectin and fenbendazole
were found to be at 3.296 min and 7.257 min, respectively. The linearity of the selected drugs was obtained in the
range of 2-6 μg/ml (ivermectin) and 60-80 μg/ml (fenbendazole). The results of analysis have been validated
according to ICH guidelines. The results for all validation parameters were found to be within the acceptance limit.
Ivermectin and fenbendazole were degraded under acidic, alkaline, oxidative, thermal and photolytic degradation
conditions. All the peaks of degraded products were well resolved from the peaks of ivermectin and fenbendazole.
The developed and validated method can be employed as a stability-indicating, as it could efficiently separate
ivermectin and fenbendazole from their degradation products.
Key words:Ivermectin, fenbendazole, HPLC, Stability indicating, Assay
Keyword :
ermectin, fenbendazole, HPLC, Stability indicating, Assay