Abstract :
Background : The major cause of pulpal and periapical diseases are living and non living irritants. The living irritants include various microorganisms including bacteria, yeast and viruses. The success of endondotic treatment depends on eradication of these microorganisms and prevention of reinfection. Mechanical instrumentation alone does not result in microorganism free root canal system, due to complex root canal anatomy. Thus, irrigants are essential to ensure microbial minimization and elimination of
organic tissue remnants. Qmix irrigating solution used as a final rinse after bleach for one step smear layer removal and disinfection and eliminate greater than 99.99% of planktonic bacteria including the resistant species E.faecalis.
Objective: The aim of this invitro study is to evaluate the antimicrobial efficacy of qmix irrigant in infected root canal system.
Material and Methods: Crowns of 60 extracted premolar teeth were sectioned at the cemento-enamel junction. The samples were stored temporarily in physiological saline solution. The canals were shaped using crown down technique upto F3 protaper file. Enterococcus faecalis was inoculated on Brain heart infusion (BHI) agar plate and incubated anaerobically at 37 ?C for 24 h. After incubation, the specimens were removed and then rinsed. In the negative group (n=20), there was no treatment. In the other group (n=20), the sections were irrigated with 5 mL of 5.25% NaOCl for 60 s. The third group (n=20) is treated with Qmix solution for 60-90 seconds. After the disinfection procedures, samples were subjected to CFUcounting evaluations.
Results: After irrigation, the elimination of bacteria and the smear layer in the positive group were significantly superior, compared with the negative group (p
Conclusion: Both irrigating solution groups, especially the Qmix group, showed satisfactory bactericidal effects in experimentally contaminated root canals.
Keyword :
Qmix, E.faecalis.